Personally I don't see the point of Chalice of Life. It's 3cc (slow), dies to Abrupt Decay like anything else, can't be protected by Mother, and only deals 5 damage per turn. Ascendant is slightly more fragile but costs 1 and is a 12-point life swing. I'd rather just use Recruiter of the Guard (to have more tutors for Ranger / Martyr / Serra) than try to play Chalice of Life, but I worked Chalice into your decks as requested.
4 Mother of Runes
4 Serra Ascendant
4 Martyr of Sands
3 Soul Warden
3 Soul Attendant
4 Ajani's Pridemate
4 Squadron Hawk
3 Stoneforge Mystic
2 Serra Avenger
3 Recruiter of the Guard
2 Ranger of Eos
4 Swords to Plowshares
//Artifacts: 2
1 Sword of Light and Shadow
1 Umezawa's Jitte
//Lands: 18
3 Karakas
1 Ancient Den
14 Plains
3 Thalia, Guardian of Thraben
3 Enlightened Tutor
1 Grafdigger's Cage
1 Rest in Peace
1 Spirit of the Labyrinth
1 Eidolon of Rhetoric
1 Containment Priest
1 Phyrexian Revoker
1 Ethersworn Canonist
2 Chalice of Life
If you want to cut out the Sisters part, you could cram in more maindeck hatebears:
4 Mother of Runes
4 Serra Ascendant
4 Martyr of Sands
4 Judge's Familiar
4 Squadron Hawk
3 Thalia, Guardian of Thraben
3 Stoneforge Mystic
2 Thalia, Heretic Cathar
2 Ranger of Eos
4 Swords to Plowshares
3 Enlightened Tutor
//Enchantments: 2
1 Oblivion Ring
1 Rest in Peace
//Artifacts: 3
1 Sword of Light and Shadow
1 Umezawa's Jitte
1 Chalice of Life
//Lands: 18
3 Karakas
1 Ancient Den
14 Plains
2 Rest in Peace
2 Helm of Obedience
2 Recruiter of the Guard
2 Containment Priest
2 Phyrexian Revoker
1 Grafdigger's Cage
1 Spirit of the Labyrinth
1 Eidolon of Rhetoric
1 Ethersworn Canonist
1 Chalice of Life
Personally I don't see the point of Chalice of Life. It's 3cc (slow), dies to Abrupt Decay like anything else, can't be protected by Mother, and only deals 5 damage per turn. Ascendant is slightly more fragile but costs 1 and is a 12-point life swing. I'd rather just use Recruiter of the Guard (to have more tutors for Ranger / Martyr / Serra) than try to play Chalice of Life, but I worked Chalice into your decks as requested.